We discuss the changes in hydrological characteristics of water bodies caused by freshwater withdrawals to meet the interests of various users on the territory of the Syunik Province of the Republic of Armenia. An analysis is made of the possibilities of combining the water withdrawal for mining enterprises and for agriculture. The problems with water supply of settlements located on this territory are highlighted. The temporal dynamics of water withdrawals is presented for separate rivers as well as its relationship with the minimum and ecological flow. It is found that the anthropogenic load on water bodies is more clearly pronounced in the case of a degradation of the quality of water resources as a result of the industrial waste water diversion, and wash-out of pollutants from the territory of enterprises during floods and in the case of emergency input of wastes from tailings pools, rock spoil heaps and waste storage areas. Results of these authors’ observations, data of routine observations and material from published sources were used in analyzing the water quality in the rivers of the Syunik Province. We calculated the coefficients of ecological quality and determined the river water quality classes according to physicochemical and biological indicators. Assessments of the river water quality class were made in accordance with the methodology of Directive 2000/60/EC. We identified the main pollutants that are responsible for the water quality class, and the factors of impact on natural waters governing the ecological status of water bodies. A comprehensive analysis is made of the intensity of anthropogenic impact on surface waters. The ecological status of water bodies is determined. Spatial changes in the quality indicators of the water of the region’s main rivers are demonstrated. Special emphasis is placed on the need to implement immediate measures to improve technological processes and bring the operation of enterprises into compliance with ecological normative standards.Keywords: ecological standardization, overland runoff, water resources management, water use, water quality.